Sepsis/Meningitis

EuSep Screen® Lattanti

REF

9144

Description

Real time PCR test for the detection of bacterial DNA belonging to Klebsiella pneumoniae, Escherichia coli, Streptococcus agalactiae (Streptococcus B or GBS) and Listeria monocytogenes.

Packaging

8 test

Certification

“Sepsis is a rare complication of an infection, the consequences of which can be very serious and potentially fatal. It consists of an excessive inflammatory response of the body to a generalized infection that damages tissues and organs, compromising their functioning” – Istituto Superiore di Sanità (ISS) – 2019
Molecular diagnostic tools enable the pathogens identification in a short time and therefore they can limit the use of broad-spectrum antibiotics, which may be un-effective. EuSep Screen® Lattanti is able to detect non-viable pathogen as a consequence of antibiotics administration before the sample’s collection

Summary Documents Related products Information Request

Summary

Technology

Real Time PCR technology

Target

Group B Streptococcus
Klebsiella pneumoniae
Escherichia coli
Listeria monocytogenes

Genetic region

gene phoE (Klebsiella pneumoniae all serotypes)
gene uidB (Escherichia coli, all serotypes)
gene sip (Streptococcus agalactiae, all serotypes)
gene iap (Listeria monocytogenes, meningites related strain)

Mastermix number

Internal control

Exogenous

Fluorophores

Limit of Detection (LoD)

2 copies/µl (10 copies/reaction)

Analytical specificity

A wide range of pathogens was used to test the assay. No cross-reactivity was found.

Diagnostic sensitivity

100 %

Diagnostic specificity

100 %

Specimen

Whole blood
CSF
Pleural fluid
Synovial fluid
Other normally sterile human biological material from newborn

Validated thermalcyclers

Applied Biosystems™ 7300 and 7500, 7500 Fast e 7500 Fast Dx
Step One e Step One Plus Real-time PCR Instrument system (Thermo Fisher Scientific)
CFX96 Touch, CFX Connect, CFX Opus 96 Real-time PCR detection system (Bio-Rad)
Eppendorf RealPlex
Qiagen Rotor-Gene 3000/6000

Software

Easy-NAT