Portfolio · Diagnostic tests

Respiratory Infections

NeoPlex™ TB/NTM-5 Detection

REF

9429

Description

Real-time PCR assay for the detection of TB and 5 pathogenic NTM in a single tube

Packaging

96 test

Certification

Tuberculosis is one of the most world’s threatening infectious diseases caused by Mycobacterium tuberculosis (TB) with high morbidity and mortality. Non-tuberculosis mycobacteria (NTM) infections, particularly with clinically relevant NTM species, are increasing in countries with low TB prevalence. Since therapeutic regimens are chosen according to the respective mycobacteria species, the reliable identification of TB and NTM is crucial for the treatment and prevention measures.

Summary

Technology

Multiplexing technology

Target

  • Mycobacterium tuberculosis
  • Mycobacterium avium
  • Mycobacterium intracellulare
  • Mycobacterium kansasii
  • Mycobacterium abscessus
  • Mycobacterium massiliense

Mastermix number

1

Internal control

Endogenous

UDG

Yes

Fluorophores

  • FAM
  • HEX
  • Cal Red 610
  • Quasar 670

Analytical specificity

A wide range of pathogens was used to test the assay. No cross-reactivity was found.

Diagnostic sensitivity

80%

Diagnostic specificity

100%

Riproducibility

100%

Specimen

Sputum

Validated thermalcyclers

CFX96™ Real-time PCR detection system (Bio-Rad)

Software

NeoPlex Viewer/Easy-NAT

Documents

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